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Image Search Results
Journal: Journal of Translational Medicine
Article Title: Niraparib exhibits a synergistic anti-tumor effect with PD-L1 blockade by inducing an immune response in ovarian cancer
doi: 10.1186/s12967-021-03073-0
Figure Lengend Snippet: The information of antibodies used for western blot, immunohistochemistry, and flow cytometry
Article Snippet: CD3 and isotype , Human , 5 μl of antibody per test ,
Techniques: Western Blot, Immunohistochemistry, Flow Cytometry
Journal: bioRxiv
Article Title: Selective Immune Silencing by Targeted TGF-β Agonists
doi: 10.64898/2026.01.19.700410
Figure Lengend Snippet: a. Dose–response curves showing pSMAD2/3 levels in human CD4- or CD8α-transduced HEK293 cells measured using a pSMAD–induced SEAP assay. b. Dose-dependent curves showing numbers of in vitro –cultured CD4 or CD8 T cells treated with the indicated proteins on day 5. c. Schematic of the experimental design for human spleen organoid culture, LAIV stimulation, and protein treatments. d. Representative flow cytometry plots showing percentages of CD38 Ki-67 cells in CD4 or CD8 T cells from the indicated treatment groups. e. Quantification of CD38 Ki-67 cell percentages in CD4 or CD8 T cells from the indicated treatment groups. f. Quantification of CD38 Ki-67 CD4 or CD8 T cell percentages among total CD3 T cells from the indicated treatment groups. g. Dose–response curves showing pSMAD2/3 levels in human CD19-transduced HEK293 cells measured using a pSMAD-induced SEAP assay. h. Representative flow cytometry plots showing CD38 high PB percentages in B cells from the indicated treatment groups. i. Quantification of CD38 high PB percentages among total leukocytes from the indicated treatment groups. j. Quantification of CD38 expression levels in CD38 low non-PB from the indicated treatment groups. k. Quantification of flu-specific IgG levels in culture supernatants from the indicated treatment groups on days 4 and 7. Data are presented as mean ± s.e.m. The statistics were obtained by one-way ANOVA coupled with Dunnett’s multiple-comparisons test (e, f, and i-k) .
Article Snippet: For human in vitro cultures, T cells were plated at 0.75 × 10 6 cells/mL in flat-bottom 12-well plates coated overnight with 5 μg/mL
Techniques: SEAP Assay, In Vitro, Cell Culture, Flow Cytometry, Expressing