anti human cd3 Search Results


human cd3  (Miltenyi Biotec)
96
Miltenyi Biotec human cd3
Human Cd3, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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k rat polyclonal anti human anti cd3  (Bio-Rad)
96
Bio-Rad k rat polyclonal anti human anti cd3
K Rat Polyclonal Anti Human Anti Cd3, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti cd3  (Cytek Biosciences)
93
Cytek Biosciences anti cd3
Anti Cd3, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti cd3 biotin  (Miltenyi Biotec)
96
Miltenyi Biotec anti cd3 biotin
Anti Cd3 Biotin, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd3 apc miltenyi 130 113 125 bw264  (Miltenyi Biotec)
95
Miltenyi Biotec cd3 apc miltenyi 130 113 125 bw264
Cd3 Apc Miltenyi 130 113 125 Bw264, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mca463gt  (Bio-Rad)
93
Bio-Rad mca463gt
Key Resources Table
Mca463gt, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti human cd3  (Proteintech)
93
Proteintech anti human cd3
Generation and identification of CAR gene-modified CIK cells. ( a ) Schematic representation of the anti-h5T4 CAR construct and the control CAR construct. The 5T4-28Z CAR contained, in sequence from the N-terminus to the C-terminus, a CD8α signal peptide (SP), an anti-h5T4 scFv comprising the variable regions of the heavy chain (VH) and light chain (Vκ) fused with a linker, a myc-tag sequence, a CD8α hinge, the CD28 transmembrane (TM) and intracellular (IC) regions followed by the CD3ζ signalling domain. The control CAR comprised a sequence identical to 5T4-28Z except without the scFv region. ZsGreen was used as a reporter gene. ( b ) CIK cells were generated as mentioned in the Methods section. They were transduced with CAR-encoded lentivirus and sorted using FACS according to the GFP expression. The surface expression of CARs on CIK cells was verified using FACS with an Alexa Fluor ® 647-conjugated myc-tag antibody. As controls, matched isotype antibody staining was incorporated. Flow plots are representative of quintuplicate cultures. ( c ) Brightfield and fluorescence images of sorted 5T4-28Z CAR- or C-28Z CAR-expressing CIK cells were captured. Representative images and merged images are displayed.
Anti Human Cd3, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti cd3 antibody  (Miltenyi Biotec)
95
Miltenyi Biotec anti cd3 antibody
Generation and identification of CAR gene-modified CIK cells. ( a ) Schematic representation of the anti-h5T4 CAR construct and the control CAR construct. The 5T4-28Z CAR contained, in sequence from the N-terminus to the C-terminus, a CD8α signal peptide (SP), an anti-h5T4 scFv comprising the variable regions of the heavy chain (VH) and light chain (Vκ) fused with a linker, a myc-tag sequence, a CD8α hinge, the CD28 transmembrane (TM) and intracellular (IC) regions followed by the CD3ζ signalling domain. The control CAR comprised a sequence identical to 5T4-28Z except without the scFv region. ZsGreen was used as a reporter gene. ( b ) CIK cells were generated as mentioned in the Methods section. They were transduced with CAR-encoded lentivirus and sorted using FACS according to the GFP expression. The surface expression of CARs on CIK cells was verified using FACS with an Alexa Fluor ® 647-conjugated myc-tag antibody. As controls, matched isotype antibody staining was incorporated. Flow plots are representative of quintuplicate cultures. ( c ) Brightfield and fluorescence images of sorted 5T4-28Z CAR- or C-28Z CAR-expressing CIK cells were captured. Representative images and merged images are displayed.
Anti Cd3 Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti cd3 apc  (Miltenyi Biotec)
94
Miltenyi Biotec anti cd3 apc
Generation and identification of CAR gene-modified CIK cells. ( a ) Schematic representation of the anti-h5T4 CAR construct and the control CAR construct. The 5T4-28Z CAR contained, in sequence from the N-terminus to the C-terminus, a CD8α signal peptide (SP), an anti-h5T4 scFv comprising the variable regions of the heavy chain (VH) and light chain (Vκ) fused with a linker, a myc-tag sequence, a CD8α hinge, the CD28 transmembrane (TM) and intracellular (IC) regions followed by the CD3ζ signalling domain. The control CAR comprised a sequence identical to 5T4-28Z except without the scFv region. ZsGreen was used as a reporter gene. ( b ) CIK cells were generated as mentioned in the Methods section. They were transduced with CAR-encoded lentivirus and sorted using FACS according to the GFP expression. The surface expression of CARs on CIK cells was verified using FACS with an Alexa Fluor ® 647-conjugated myc-tag antibody. As controls, matched isotype antibody staining was incorporated. Flow plots are representative of quintuplicate cultures. ( c ) Brightfield and fluorescence images of sorted 5T4-28Z CAR- or C-28Z CAR-expressing CIK cells were captured. Representative images and merged images are displayed.
Anti Cd3 Apc, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd3 percp  (Miltenyi Biotec)
95
Miltenyi Biotec cd3 percp
Generation and identification of CAR gene-modified CIK cells. ( a ) Schematic representation of the anti-h5T4 CAR construct and the control CAR construct. The 5T4-28Z CAR contained, in sequence from the N-terminus to the C-terminus, a CD8α signal peptide (SP), an anti-h5T4 scFv comprising the variable regions of the heavy chain (VH) and light chain (Vκ) fused with a linker, a myc-tag sequence, a CD8α hinge, the CD28 transmembrane (TM) and intracellular (IC) regions followed by the CD3ζ signalling domain. The control CAR comprised a sequence identical to 5T4-28Z except without the scFv region. ZsGreen was used as a reporter gene. ( b ) CIK cells were generated as mentioned in the Methods section. They were transduced with CAR-encoded lentivirus and sorted using FACS according to the GFP expression. The surface expression of CARs on CIK cells was verified using FACS with an Alexa Fluor ® 647-conjugated myc-tag antibody. As controls, matched isotype antibody staining was incorporated. Flow plots are representative of quintuplicate cultures. ( c ) Brightfield and fluorescence images of sorted 5T4-28Z CAR- or C-28Z CAR-expressing CIK cells were captured. Representative images and merged images are displayed.
Cd3 Percp, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fitc anti human cd3  (Miltenyi Biotec)
95
Miltenyi Biotec fitc anti human cd3
Generation and identification of CAR gene-modified CIK cells. ( a ) Schematic representation of the anti-h5T4 CAR construct and the control CAR construct. The 5T4-28Z CAR contained, in sequence from the N-terminus to the C-terminus, a CD8α signal peptide (SP), an anti-h5T4 scFv comprising the variable regions of the heavy chain (VH) and light chain (Vκ) fused with a linker, a myc-tag sequence, a CD8α hinge, the CD28 transmembrane (TM) and intracellular (IC) regions followed by the CD3ζ signalling domain. The control CAR comprised a sequence identical to 5T4-28Z except without the scFv region. ZsGreen was used as a reporter gene. ( b ) CIK cells were generated as mentioned in the Methods section. They were transduced with CAR-encoded lentivirus and sorted using FACS according to the GFP expression. The surface expression of CARs on CIK cells was verified using FACS with an Alexa Fluor ® 647-conjugated myc-tag antibody. As controls, matched isotype antibody staining was incorporated. Flow plots are representative of quintuplicate cultures. ( c ) Brightfield and fluorescence images of sorted 5T4-28Z CAR- or C-28Z CAR-expressing CIK cells were captured. Representative images and merged images are displayed.
Fitc Anti Human Cd3, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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er 170 cd3 ucht1 s fluidigm 3170001b  (fluidigm)
95
fluidigm er 170 cd3 ucht1 s fluidigm 3170001b
Generation and identification of CAR gene-modified CIK cells. ( a ) Schematic representation of the anti-h5T4 CAR construct and the control CAR construct. The 5T4-28Z CAR contained, in sequence from the N-terminus to the C-terminus, a CD8α signal peptide (SP), an anti-h5T4 scFv comprising the variable regions of the heavy chain (VH) and light chain (Vκ) fused with a linker, a myc-tag sequence, a CD8α hinge, the CD28 transmembrane (TM) and intracellular (IC) regions followed by the CD3ζ signalling domain. The control CAR comprised a sequence identical to 5T4-28Z except without the scFv region. ZsGreen was used as a reporter gene. ( b ) CIK cells were generated as mentioned in the Methods section. They were transduced with CAR-encoded lentivirus and sorted using FACS according to the GFP expression. The surface expression of CARs on CIK cells was verified using FACS with an Alexa Fluor ® 647-conjugated myc-tag antibody. As controls, matched isotype antibody staining was incorporated. Flow plots are representative of quintuplicate cultures. ( c ) Brightfield and fluorescence images of sorted 5T4-28Z CAR- or C-28Z CAR-expressing CIK cells were captured. Representative images and merged images are displayed.
Er 170 Cd3 Ucht1 S Fluidigm 3170001b, supplied by fluidigm, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Key Resources Table

Journal: Cell

Article Title: The identity of human tissue-emigrant CD8 + T cells

doi: 10.1016/j.cell.2020.11.019

Figure Lengend Snippet: Key Resources Table

Article Snippet: UCTH1 [anti-CD3] , Bio-Rad , MCA463GT (RRID:AB_1101798).

Techniques: Western Blot, Isolation, Recombinant, Lysis, Purification, Sequencing, Gene Expression, Software

Generation and identification of CAR gene-modified CIK cells. ( a ) Schematic representation of the anti-h5T4 CAR construct and the control CAR construct. The 5T4-28Z CAR contained, in sequence from the N-terminus to the C-terminus, a CD8α signal peptide (SP), an anti-h5T4 scFv comprising the variable regions of the heavy chain (VH) and light chain (Vκ) fused with a linker, a myc-tag sequence, a CD8α hinge, the CD28 transmembrane (TM) and intracellular (IC) regions followed by the CD3ζ signalling domain. The control CAR comprised a sequence identical to 5T4-28Z except without the scFv region. ZsGreen was used as a reporter gene. ( b ) CIK cells were generated as mentioned in the Methods section. They were transduced with CAR-encoded lentivirus and sorted using FACS according to the GFP expression. The surface expression of CARs on CIK cells was verified using FACS with an Alexa Fluor ® 647-conjugated myc-tag antibody. As controls, matched isotype antibody staining was incorporated. Flow plots are representative of quintuplicate cultures. ( c ) Brightfield and fluorescence images of sorted 5T4-28Z CAR- or C-28Z CAR-expressing CIK cells were captured. Representative images and merged images are displayed.

Journal: Scientific Reports

Article Title: 5T4-specific chimeric antigen receptor modification promotes the immune efficacy of cytokine-induced killer cells against nasopharyngeal carcinoma stem cell-like cells

doi: 10.1038/s41598-017-04756-9

Figure Lengend Snippet: Generation and identification of CAR gene-modified CIK cells. ( a ) Schematic representation of the anti-h5T4 CAR construct and the control CAR construct. The 5T4-28Z CAR contained, in sequence from the N-terminus to the C-terminus, a CD8α signal peptide (SP), an anti-h5T4 scFv comprising the variable regions of the heavy chain (VH) and light chain (Vκ) fused with a linker, a myc-tag sequence, a CD8α hinge, the CD28 transmembrane (TM) and intracellular (IC) regions followed by the CD3ζ signalling domain. The control CAR comprised a sequence identical to 5T4-28Z except without the scFv region. ZsGreen was used as a reporter gene. ( b ) CIK cells were generated as mentioned in the Methods section. They were transduced with CAR-encoded lentivirus and sorted using FACS according to the GFP expression. The surface expression of CARs on CIK cells was verified using FACS with an Alexa Fluor ® 647-conjugated myc-tag antibody. As controls, matched isotype antibody staining was incorporated. Flow plots are representative of quintuplicate cultures. ( c ) Brightfield and fluorescence images of sorted 5T4-28Z CAR- or C-28Z CAR-expressing CIK cells were captured. Representative images and merged images are displayed.

Article Snippet: The following antibodies were used: anti-hROR1 polyclonal antibody (R&D), anti-hROR1-APC antibody (R&D), anti-h5T4-APC antibody (R&D), anti-h5T4 mAb (clone EPR5529, Abcam), anti-E-cadherin mAb (clone EP700Y, Abcam), anti-human N-cadherin mAb (clone EPR1791-4, Abcam), anti-human Nanog mAb (clone EPR2027(2), Abcam), anti-human Oct4 mAb (clone EPR2054, Abcam), anti-human vimentin mAb (clone EPR3776, Abcam), anti-human sox2 mAb (clone EPR3131, Abcam), anti-human β-catenin mAb (clone E247, Abcam), anti-human Carbonic Anhydrase IX polyclonal antibody (GeneTex), anti-human GAPDH antibody (Proteintech), HRP-conjugated secondary antibodies (Proteintech), diverse fluorochrome-conjugated anti-human CD3, CD8, CD56, NKG2D, CD57, PD-1, CD107α and isotype control antibodies (Pharmingen, BD).

Techniques: Modification, Construct, Control, Sequencing, Generated, Transduction, Expressing, Staining, Fluorescence

CIK cells maintain their original phenotypes after transduction of CAR genes. The expression of CD3, CD8, CD56, NKG2D, CD57, and PD-1 on CAR-engineered CIK cells and NT-CIK cells was monitored weekly using FACS with diverse fluorochrome-conjugated antibodies or matched isotype control antibodies. ( a ) Characteristic flow plots showing the expression of phenotypic markers in 5T4-28Z-CIK cells on day 7, 14, and 21 of cultivation are presented. ( b ) Histograms show the mean values ± SD of three independent experiments.

Journal: Scientific Reports

Article Title: 5T4-specific chimeric antigen receptor modification promotes the immune efficacy of cytokine-induced killer cells against nasopharyngeal carcinoma stem cell-like cells

doi: 10.1038/s41598-017-04756-9

Figure Lengend Snippet: CIK cells maintain their original phenotypes after transduction of CAR genes. The expression of CD3, CD8, CD56, NKG2D, CD57, and PD-1 on CAR-engineered CIK cells and NT-CIK cells was monitored weekly using FACS with diverse fluorochrome-conjugated antibodies or matched isotype control antibodies. ( a ) Characteristic flow plots showing the expression of phenotypic markers in 5T4-28Z-CIK cells on day 7, 14, and 21 of cultivation are presented. ( b ) Histograms show the mean values ± SD of three independent experiments.

Article Snippet: The following antibodies were used: anti-hROR1 polyclonal antibody (R&D), anti-hROR1-APC antibody (R&D), anti-h5T4-APC antibody (R&D), anti-h5T4 mAb (clone EPR5529, Abcam), anti-E-cadherin mAb (clone EP700Y, Abcam), anti-human N-cadherin mAb (clone EPR1791-4, Abcam), anti-human Nanog mAb (clone EPR2027(2), Abcam), anti-human Oct4 mAb (clone EPR2054, Abcam), anti-human vimentin mAb (clone EPR3776, Abcam), anti-human sox2 mAb (clone EPR3131, Abcam), anti-human β-catenin mAb (clone E247, Abcam), anti-human Carbonic Anhydrase IX polyclonal antibody (GeneTex), anti-human GAPDH antibody (Proteintech), HRP-conjugated secondary antibodies (Proteintech), diverse fluorochrome-conjugated anti-human CD3, CD8, CD56, NKG2D, CD57, PD-1, CD107α and isotype control antibodies (Pharmingen, BD).

Techniques: Transduction, Expressing, Control